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Factors affecting the rate of movement

Structure and functions in living organisms · Movement of substances into and out of cells · note 4 of 5

Factors affecting the rate of movementSpec 2.16

In short

Four factors affect how fast substances move into and out of cells: surface area to volume ratio, distance, temperature and concentration gradient. A larger surface area to volume ratio, a shorter distance, a higher temperature and a steeper concentration gradient all increase the rate. As an organism gets bigger, its surface area to volume ratio gets smaller.

Four factors affect how fast substances move into and out of cells by diffusion and osmosis.

Factors affecting the rate of movement
FactorEffect on the rateExample
Surface area to volume ratioA larger ratio means a faster rate for the size of the cell, because more surface is available for each unit of volumeRoot hair cells have a long projection that gives a large surface area
DistanceA shorter distance means a faster rateThe wall of an alveolus is one cell thick
TemperatureA higher temperature means a faster rate, because particles have more kinetic energy and move fasterDiffusion is faster in a warm solution than in a cold one
Concentration gradientA steeper gradient (a bigger difference in concentration) means a faster rateBlood flow keeps the oxygen concentration lower in the blood than in the alveoli

Surface area to volume ratio

The surface area : volume ratio tells you how much surface an organism or cell has compared with its size. The bigger the object, the smaller this ratio becomes.

surface area : volume ratio = surface area ÷ volume

Surface area : volume ratio of a cube

A cube-shaped block of tissue has sides of 3 cm. Calculate its surface area : volume ratio.

  1. A cube has 6 faces. Area of one face = 3 × 3 = 9 cm².
  2. Surface area = 6 × 9 = 54 cm².
  3. Volume = 3 × 3 × 3 = 27 cm³.
  4. Ratio = 54 : 27. Divide both sides by 27.

Answer: 2 : 1

Three cubes drawn to scale with sides of 1 cm, 2 cm and 3 cm, labelled with surface areas of 6, 24 and 54 cm², volumes of 1, 8 and 27 cm³, and surface area : volume ratios of 6 : 1, 3 : 1 and 2 : 1. (opens full size in a new tab)
As the cube gets bigger, its surface area : volume ratio gets smaller.
  • A single-celled organism has a large surface area : volume ratio. Substances only have a short distance to diffuse, so diffusion across the surface is fast enough.
  • A multicellular organism has a small surface area : volume ratio. Many cells are deep inside the body, so the diffusion distance is long and diffusion across the body surface is too slow.
Common mistake:

A thin membrane or wall gives a short diffusion distance. It does not give a larger surface area: these are two separate factors.

Written and checked against the Edexcel IGCSE Science Double Award (4SD0) specification · Updated October 2026

Frequently asked questions

How does osmosis differ from diffusion?

Osmosis is a special type of diffusion that involves only water moving through a partially permeable membrane. Diffusion is the net movement of any particles, such as oxygen or carbon dioxide, from a higher to a lower concentration. In osmosis, water moves from a dilute solution to a more concentrated solution. Neither process needs energy from respiration.

Why does osmosis not require energy?

Osmosis does not require energy because it is a passive process. Water simply diffuses from a dilute solution to a more concentrated solution through a partially permeable membrane, so no energy from respiration is used. Only active transport, which moves substances against a concentration gradient, needs energy from respiration.

Why is active transport needed in plant roots?

Active transport is needed in plant roots because root hair cells often contain a higher concentration of mineral ions than the soil. Diffusion would move ions out of the cell, so the cells must use energy from respiration to move ions in against the concentration gradient. This lets plants take in ions that are in short supply.

All 5 questions on Movement of substances into and out of cells