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Rate calculations for enzyme activity

Key concepts in biology · Enzyme action and activity · note 5 of 5

Rate calculations for enzyme activitySpec 1.11

In short

The rate of reaction is how fast a reaction happens, calculated as the amount of product formed divided by the time taken. Its units depend on what is measured, such as cm³/s for a volume of gas. If the time for a reaction to finish is measured, the rate is 1 ÷ time, in s⁻¹.

The rate of reaction tells you how fast a reaction is happening. It is a compound measure, because it combines an amount with a time.

rate = amount of product formed ÷ time taken

The units depend on what you measure, for example cm³/s for a volume of gas, or g/min for a mass. If an experiment measures the time taken for a reaction to finish, such as the time for starch to disappear, the rate can be found as 1 ÷ time, with units of s⁻¹.

Calculating a rate

An enzyme produces 12 cm³ of gas in 40 seconds. Calculate the rate of reaction in cm³/s.

  1. rate = amount of product ÷ time
  2. 12 ÷ 40 = 0.3

Answer: 0.3 cm³/s

Maths skill:

A longer time means a slower reaction. If the time doubles, the rate (1 ÷ time) halves.

Quick check

  1. What is the name of the part of an enzyme that the substrate fits into?

    Show answer

    The active site.

  2. Why does an enzyme stop working at a high temperature?

    Show answer

    The shape of the active site changes, so the substrate no longer fits. The enzyme is denatured.

  3. Why does the rate level off as substrate concentration increases?

    Show answer

    All the active sites are occupied.

  4. In the pH core practical, what colour shows that starch is still present with iodine solution?

    Show answer

    Blue-black.

  5. Calculate the rate when 30 cm³ of gas is collected in 60 s.

    Show answer

    0.5 cm³/s.

Written and checked against the Edexcel GCSE Combined Science (1SC0) specification · Updated October 2026

Frequently asked questions

How do enzymes speed up reactions?

Enzymes speed up reactions by acting as biological catalysts, without being used up. The substrate collides with the enzyme's active site and fits into it because their shapes are complementary, forming an enzyme-substrate complex. The reaction happens at the active site, the products leave, and the enzyme can be used again.

Why do enzymes denature at high temperatures?

Enzymes denature at high temperatures because the bonds that hold the enzyme in shape are broken. The enzyme and its active site change shape, so the substrate no longer fits and the reaction cannot be catalysed. This change is permanent, so above the optimum temperature the rate falls quickly towards zero.

What happens to enzymes at low temperatures?

At low temperatures enzymes work slowly, but they are not denatured. The particles have less kinetic energy and move more slowly, so the enzyme and substrate collide less often and the rate of reaction is lower. As the temperature rises towards the optimum, collisions become more frequent and the rate increases.

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