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Rate calculations for transpiration

Plant structures and their functions · Leaves, water uptake and extreme environments · note 2 of 2

Rate calculations for transpirationSpec 6.13

In short

A rate of transpiration or water uptake is the change in a quantity divided by the time taken, for example the distance a bubble moves in a potometer per minute. Percentage change in mass equals change in mass divided by original mass, times 100. Calculate a mean from repeats first, and always give the unit, such as mm per minute.

A rate tells you how much something changes in a unit of time. For water uptake or transpiration you can use the distance moved by a bubble in a potometer, the volume of water lost, or the change in mass of a plant.

rate = change in quantity ÷ time taken
percentage change = (change in mass ÷ original mass) × 100

Rate of water uptake

In a potometer, an air bubble moved 31 mm, 28 mm and 34 mm in three repeats of 10 minutes each. Calculate the mean rate of water uptake in mm per minute.

  1. Mean distance = (31 + 28 + 34) ÷ 3 = 93 ÷ 3 = 31 mm.
  2. Rate = distance ÷ time = 31 ÷ 10 = 3.1 mm per minute.

Answer: 3.1 mm per minute

Percentage loss of mass

A leafy shoot had a mass of 25.0 g at the start of an investigation and 23.0 g at the end. Calculate the percentage loss of mass.

  1. Loss of mass = 25.0 − 23.0 = 2.0 g.
  2. Percentage loss = 2.0 ÷ 25.0 × 100 = 8%.

Answer: 8%

Maths skill:

Always include the unit with a rate, for example mm per minute or cm³ per hour. Calculate a mean first if you are given repeats.

Quick check

  1. What does a potometer measure?

    Show answer

    The rate of water uptake of a leafy shoot.

  2. Why does moving air increase the rate of transpiration?

    Show answer

    It carries away water vapour, keeping a steep concentration gradient so water vapour diffuses out faster.

  3. A bubble in a potometer moves 24 mm in 8 minutes. What is the rate?

    Show answer

    24 ÷ 8 = 3 mm per minute.

  4. Why does a higher temperature increase the rate of water uptake?

    Show answer

    Water evaporates from the leaf cells faster and water vapour diffuses out faster, so more water is pulled up the xylem.

Written and checked against the Edexcel GCSE Combined Science (1SC0) specification · Updated October 2026

Frequently asked questions

How does light intensity affect the rate of transpiration?

A higher light intensity increases the rate of transpiration. The stomata open in the light, so more water vapour can diffuse out of the leaf, and more water is pulled up through the xylem. In low light or darkness the stomata close, so less water vapour is lost and the rate of water uptake falls.

How does a potometer work?

A potometer measures the rate of water uptake of a leafy shoot. As the shoot takes up water, an air bubble moves along a capillary tube. The distance the bubble moves in a set time is a measure of the rate. You can compare conditions, for example with and without a fan, or in the light and in the shade.

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