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Changes in microscope technology

Key concepts in biology · Microscopy and measuring cells · note 1 of 4

Changes in microscope technologySpec 1.3

In short

An electron microscope uses a beam of electrons and has a much higher magnification and resolution than a light microscope. Resolution is how well two points close together can be seen as separate points. Because of this, sub-cellular structures such as ribosomes and the inside of mitochondria can be seen in detail, which has increased understanding of their functions.

Early light microscopes could show cells, but not much detail inside them. Two ideas matter here. Magnification is how many times bigger the image is than the real object. Resolution (resolving power) is how well a microscope can show two points that are close together as separate points.

Light and electron microscopes compared
Light microscopeElectron microscope
UsesLight and glass lensesA beam of electrons
MagnificationLowerMuch higher
ResolutionLowerMuch higher
What can be seenCells, nucleus, and some larger structures such as chloroplastsFine detail, such as the internal structure of mitochondria and chloroplasts, and ribosomes

Because electron microscopes have a higher magnification and higher resolution, we can see sub-cellular structures in much more detail and with more clarity than in the past. This has increased our understanding of what each structure does, because we can now see how its structure is related to its function.

Exam tip:

For a full answer to 'explain how electron microscopes have increased understanding', say three things: higher magnification and resolution, so more detail can be seen, which helps us understand the role of sub-cellular structures.

Written and checked against the Edexcel GCSE Combined Science (1SC0) specification · Updated October 2026

Frequently asked questions

How do you calculate magnification in biology?

Magnification is calculated using magnification = size of image ÷ size of real object. Both sizes must be in the same unit, so convert first, for example from mm to µm by multiplying by 1000. To find the real size, rearrange to real size = image size ÷ magnification. Total microscope magnification is eyepiece × objective.

What is the difference between magnification and resolution?

Magnification is how many times bigger the image is than the real object, while resolution is how well a microscope can show two points close together as separate points. Electron microscopes have a much higher magnification and resolution than light microscopes, so they show fine detail such as ribosomes and the inside of mitochondria.

How many micrometres are in a millimetre?

There are 1000 micrometres (µm) in 1 millimetre. Each unit in the sequence millimetre, micrometre, nanometre and picometre is 1000 times smaller than the one before. To convert to a smaller unit, multiply by 1000; to convert to a larger unit, divide by 1000. For example, 0.2 mm is 200 µm.

All 4 questions on Microscopy and measuring cells