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Measuring the energy in food

Key concepts in biology · Biological catalysts, food tests and energy in food · note 3 of 3

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In short

Calorimetry measures the energy in food by burning it to heat a known mass of water and recording the temperature rise. Energy released (J) = mass of water (g) × 4.2 × temperature change (°C), and dividing by the mass of food gives the energy per gram. The result is usually too low, because heat is lost to the surroundings.

Food contains stored chemical energy. Energy can be measured using calorimetry: the food is burned and the energy it releases is used to heat a known mass of water.

  1. Measure a known volume of water into a boiling tube and record its starting temperature. 1 cm³ of water has a mass of 1 g.
  2. Weigh a small piece of food and hold it on a mounted needle.
  3. Set fire to the food and hold it under the boiling tube until the food stops burning.
  4. Record the highest temperature of the water and work out the temperature change.
energy released (J) = mass of water (g) × 4.2 × temperature change (°C)
energy per gram of food (J/g) = energy released ÷ mass of food (g)

Calculating the energy in food

Burning 0.5 g of a snack raises the temperature of 20 g of water by 12 °C. Calculate the energy released per gram of the snack.

  1. energy released = 20 × 4.2 × 12 = 1008 J
  2. energy per gram = 1008 ÷ 0.5 = 2016 J/g

Answer: 2016 J/g

The result is usually lower than the real value because heat is lost to the surroundings and the food may not burn completely. Surrounding the boiling tube with a draught shield, putting the flame close to the tube and stirring the water improve the results. Wear eye protection, take care with the burning food, and do not use foods such as nuts that people may be allergic to.

Quick check

  1. What are proteins broken down into?

    Show answer

    Amino acids.

  2. What does a catalyst do?

    Show answer

    It speeds up a reaction and is not used up.

  3. Which reagent turns purple when protein is present?

    Show answer

    Biuret reagent (Triple only).

  4. How can the energy in food be measured?

    Show answer

    By burning it to heat water and using the temperature change (calorimetry) (Triple only).

Written and checked against the Edexcel GCSE Biology (1BI0) specification · Updated October 2026

Frequently asked questions

Why are enzymes important in the body?

Enzymes are important because chemical reactions in living things would be far too slow to keep us alive without them. As biological catalysts, they speed up reactions in cells. They are needed both to break down large molecules such as proteins, carbohydrates and lipids, and to build them up from smaller molecules.

What are carbohydrates, proteins and lipids broken down into?

Carbohydrates are broken down into sugars, proteins into amino acids, and lipids into fatty acids and glycerol. Each reaction is catalysed by a different enzyme, because enzymes are specific. The same small molecules are also used to synthesise the large carbohydrates, proteins and lipids that the body needs.

How do you test for protein in food?

Triple only To test for protein, add biuret reagent to a liquid sample of the food and mix. If protein is present, the colour changes from blue to purple (lilac). If there is no protein, it stays blue. Solid food is first crushed with a little water. Always wear eye protection during food tests.

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