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Gas exchange, diffusion and stomata

Structure and functions in living organisms · Gas exchange in flowering plants · note 1 of 3

Gas exchange, diffusion and stomataSpec 2.40B, 2.41B, 2.43B, 2.70

In short

Metabolism is all the chemical reactions in a cell, and two of its products are waste products in a plant. Carbon dioxide is a waste product of respiration, and oxygen is a waste product of photosynthesis. Both gases leave the plant from the leaves by diffusion through the stomata.

Waste products of metabolism

Metabolism is all the chemical reactions in a cell. Two of its products are waste products in a plant. Carbon dioxide is a waste product of respiration. Oxygen is a waste product of photosynthesis. Both leave the plant from the leaves by diffusion through the stomata.

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Diffusion in gas exchange

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Diffusion is the net movement of particles from a region of higher concentration to a region of lower concentration, down a concentration gradient. Gases move into and out of leaves by diffusion, so no energy is needed to move them.

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Plants use and make both gases. Gas exchange of carbon dioxide and oxygen therefore depends on two processes at the same time.

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Gas exchange in relation to respiration and photosynthesis
ProcessTakes inGives outWhen it happens
RespirationOxygenCarbon dioxideAll the time, in light and dark
PhotosynthesisCarbon dioxideOxygenOnly when there is light
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Gases reach the mesophyll cells through the stomata and the air spaces inside the leaf. How the structure of the leaf is adapted for gas exchange is covered in Leaf structure and adaptations.

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The role of stomata

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A stoma is a pore in the leaf surface. It is surrounded by two guard cells. When the guard cells take in water they swell and the stoma opens. When they lose water they become limp and the stoma closes. Carbon dioxide enters and oxygen leaves through open stomata. Water vapour is also lost through them, so the plant closes its stomata when it loses too much water.

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Surface views of a stoma: open, with swollen guard cells, carbon dioxide going in and oxygen and water vapour going out; closed, with limp guard cells and no pore. (opens full size in a new tab)
Guard cells that take in water swell and open the stoma; guard cells that lose water go limp and close it.
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Exam tip:

In the day, carbon dioxide diffuses into the leaf because photosynthesis uses it up, so its concentration inside the leaf is lower than in the air.

Written and checked against the Edexcel IGCSE Biology (4BI1) specification · Updated October 2026

Frequently asked questions

How do gases move into and out of a leaf?

Biology only Gases move into and out of a leaf by diffusion through the stomata, down their concentration gradients, so no energy is needed. In the day, carbon dioxide diffuses in because photosynthesis uses it up, lowering its concentration inside the leaf. Oxygen, a waste product of photosynthesis, diffuses out through the stomata into the air.

How do guard cells open and close stomata?

Biology only Guard cells open a stoma when they take in water and swell, and close it when they lose water and become limp. Open stomata let carbon dioxide in and oxygen out, but water vapour is also lost through them. The plant therefore closes its stomata when it is losing too much water.

Do plants respire at night and during the day?

Biology only Yes, plants respire all the time, in light and dark, but photosynthesise only when there is light. At night they release carbon dioxide and take in oxygen. In bright light photosynthesis is faster than respiration, so the carbon dioxide from respiration is used up and the plant takes in carbon dioxide overall.

All 5 questions on Gas exchange in flowering plants