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Practical: energy content of food

Structure and functions in living organisms · Human nutrition · note 7 of 7

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Practical: energy content of foodSpec 2.33B

In short

The energy in food can be compared by burning a sample under a known mass of water and measuring the temperature rise. Energy released in joules equals mass of water in grams × 4.2 × temperature change, and dividing by the mass of food gives joules per gram. Results are usually too low because heat is lost to the surroundings.

Food stores chemical energy. You can compare the energy in different foods by burning a sample and using the energy released to heat a known mass of water.

  1. Measure a known volume of water (for example 20 cm³) into a boiling tube and record its starting temperature. 1 cm³ of water has a mass of 1 g.
  2. Weigh a small piece of food and hold it on a mounted needle.
  3. Light the food in a Bunsen burner flame, then hold it under the boiling tube straight away until the food stops burning. Stir the water gently.
  4. Record the highest temperature of the water and work out the temperature change.
A boiling tube of water with a thermometer, clamped to a clamp stand above burning food held on a mounted needle, with arrows showing heat transfer to the tube, all standing on a heatproof mat. (opens full size in a new tab)
Burning food heats a known mass of water; the temperature rise is used to calculate the energy released.
energy released (J) = mass of water (g) × 4.2 × temperature change (°C)
energy per gram of food (J/g) = energy released (J) ÷ mass of food (g)

Calculating the energy in food

Burning 0.5 g of a snack raises the temperature of 20 g of water by 12 °C. Calculate the energy released per gram of the snack.

  1. energy released = 20 × 4.2 × 12 = 1008 J
  2. energy per gram = 1008 ÷ 0.5 = 2016 J/g

Answer: 2016 J/g

The result is usually lower than the real energy in the food. Heat is lost to the surroundings and the food may not burn completely. A draught shield, holding the flame close to the tube and stirring the water improve the results. Foods rich in lipid, such as nuts, usually release more energy per gram than foods rich in carbohydrate.

Practical:

Variables: the type of food is the independent variable; the temperature rise is the dependent variable. Keep the volume of water, the mass of food and the distance from the flame the same. Wear eye protection, take care with the burning food, and do not use nuts if anyone is allergic.

Quick check

  1. Name the seven components of a balanced diet.

    Show answer

    Carbohydrate, protein, lipid, vitamins, minerals, water and dietary fibre.

  2. What is peristalsis?

    Show answer

    Waves of muscle contraction that move food along the gut.

  3. What are the products of digestion by amylase and then maltase?

    Show answer

    Amylase makes maltose from starch. Maltase makes glucose from maltose.

  4. Give two roles of bile.

    Show answer

    It neutralises stomach acid and emulsifies lipids.

  5. Where is bile made and where is it stored?

    Show answer

    Made in the liver and stored in the gall bladder.

  6. Biology only How do you calculate the energy released when food is burned under water?

    Show answer

    Mass of water × 4.2 × temperature change.

Written and checked against the Edexcel IGCSE Biology (4BI1) specification · Updated October 2026

Frequently asked questions

What enzymes digest carbohydrates?

Amylase and maltase digest carbohydrates. Amylase, made in the salivary glands and the pancreas, breaks starch down into the sugar maltose. Maltase, made in the small intestine, then breaks maltose down into glucose. Glucose is small and soluble, so it can be absorbed into the blood in the small intestine and used in respiration.

What enzymes break down proteins?

Proteases break down proteins into amino acids. Proteases are made in the stomach, the pancreas and the small intestine. Pepsin is a protease in the acidic stomach and works best at about pH 2. The amino acids are absorbed into the blood and used to make new proteins for growth and repair.

What enzymes does the pancreas produce?

The pancreas produces amylase, protease and lipase and releases them into the duodenum. Amylase breaks starch into maltose, protease breaks proteins into amino acids, and lipase breaks lipids into fatty acids and glycerol. The pancreas is not part of the alimentary canal itself, but it helps with digestion.

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