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Transpiration

Structure and functions in living organisms · The need for transport and transport in plants · note 4 of 5

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In short

Transpiration is the evaporation of water from the surface of a plant, mostly from the leaves. Water evaporates from the cells inside the leaf into the air spaces, then water vapour diffuses out through the stomata. As water leaves, more is pulled up the xylem, forming the transpiration stream, which also carries mineral ions to the leaves.

Transpiration
The evaporation of water from the surface of a plant.

Most transpiration happens in the leaves. Water evaporates from the surface of the cells inside the leaf into the air spaces, then water vapour diffuses out through the stomata into the air.

  1. Water is absorbed from the soil by the root hair cells.
  2. Water moves up through the xylem to the leaves.
  3. Water evaporates from the surface of the cells in the leaf into the air spaces.
  4. Water vapour diffuses out of the leaf through the stomata.

As water leaves the leaf, more water is pulled up the xylem to replace it. This continuous flow is called the transpiration stream. It carries mineral ions from the roots to the leaves, and it supplies water for photosynthesis and keeps the cells firm.

A plant showing water taken in by root hair cells, moving up the xylem in the root, stem and leaf, then a magnified leaf section where water evaporates from cells into the air spaces and water vapour diffuses out through a stoma. (opens full size in a new tab)
The transpiration stream: water moves up the xylem, evaporates into the air spaces and diffuses out through the stomata.
Exam tip:

Water is lost by evaporation and then diffusion. Do not say that water 'escapes' or 'leaks' from the leaf.

Written and checked against the Edexcel IGCSE Biology (4BI1) specification · Updated October 2026

Frequently asked questions

Why is diffusion insufficient in multicellular organisms?

Diffusion is insufficient in multicellular organisms because they have a small surface area to volume ratio and most cells are deep inside the body. Diffusion over these long distances is too slow to supply every cell with enough oxygen and food, or to remove waste quickly. So they need transport systems, such as blood, or xylem and phloem.

What is the difference between xylem and phloem?

Xylem carries water and mineral ions upwards from the roots, while phloem carries sucrose and amino acids up or down the plant to where they are needed. Xylem is made of dead, hollow cells with walls strengthened by lignin. Phloem is made of living cells joined end to end, with sieve plates in the end walls.

How are root hair cells adapted to their function?

Biology only Root hair cells have a long, thin projection that gives a large surface area for absorbing water and mineral ions. A thin wall and membrane give a short distance for water. Cell sap more concentrated than soil water keeps water moving in by osmosis, and many mitochondria release energy for active transport of mineral ions.

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