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Constructing and using dichotomous keys

Characteristics and classification of living organisms · Characteristics and classification · note 3 of 4

Constructing and using dichotomous keysSpec 1.2.4

In short

A dichotomous key is a series of paired statements that identifies an organism. At each step you choose between two contrasting descriptions, such as 'has fur' and 'does not have fur', and each choice sends you to another step or to the organism's name. Good keys use features that can be observed and do not change, not size.

A dichotomous key is a series of paired statements that identifies an organism. 'Dichotomous' means 'divides into two'. At each step you choose between two descriptions, and each choice sends you to another step or to the name of an organism.

Using a key

A key to five animals
StepFeatureResult
1aHas feathersSparrow
1bDoes not have feathersGo to step 2
2aHas furRabbit
2bDoes not have furGo to step 3
3aHas finsTrout
3bDoes not have finsGo to step 4
4aSkin is dry and scalyLizard
4bSkin is moist and smoothFrog

To identify a frog, start at 1. It has no feathers (1b), so go to step 2. It has no fur (2b), so go to step 3. It has no fins (3b), so go to step 4. Its skin is moist and smooth (4b), so it is a frog.

Flow chart version of the five-animal dichotomous key: questions 'Has feathers?', 'Has fur?', 'Has fins?' and 'Skin dry and scaly?' each have a Yes and a No exit, ending at sparrow, rabbit, trout, lizard and frog. (opens full size in a new tab)
The same key as a flow chart. Each question has two exits, and every organism ends in its own box.

Constructing a key

  1. List the organisms and choose features that you can see and that are clearly different, such as feathers, fins or number of legs.
  2. Pick one feature that splits the organisms into two groups, so that some have it and some do not.
  3. Write the two statements as a pair. Each statement must be a clear contrast, for example 'has fur' and 'does not have fur'.
  4. Repeat with each group until every organism is on its own.
  5. Test the key by using it on each organism.
Exam tip:

Use features that can be observed and do not change, such as the number of legs. Avoid size, because it varies with age. Do not use two features in one statement.

Written and checked against the Cambridge IGCSE Biology (0610) specification · Updated October 2026

Frequently asked questions

What are the seven characteristics of living organisms?

The seven characteristics of living organisms are movement, respiration, sensitivity, growth, reproduction, excretion and nutrition, often remembered as MRS GREN. Every living organism shows all seven. A car or a flame may seem to show some of them, such as movement, but a non-living thing never shows all seven.

What is the difference between excretion and egestion?

Excretion is the removal of the waste products of metabolism and substances in excess of requirements, such as carbon dioxide and urea. Egestion is the removal of faeces. Faeces are undigested food that never entered the body's cells, so they are not metabolic waste, and removing them is not excretion.

Why are horses and donkeys different species?

Horses and donkeys are different species because their offspring, the mule, is sterile. A species is a group of organisms that can reproduce to produce fertile offspring. Horses and donkeys look similar and can mate, but the mule cannot produce offspring of its own, so the definition is not met.

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