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Transpiration pull and wilting

Transport in plants · Transpiration · note 3 of 3

Spec 8.3.5, 8.3.7
Supplement (what this means)Supplement: only for the Extended papers (2 and 4). Core students can skip it. What the labels mean
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Transpiration pull and wiltingSpec 8.3.5, 8.3.7

In short

Transpiration pull is the pull created when water evaporates from mesophyll cells and is replaced by water drawn out of the xylem. Water molecules are attracted to each other, so they form a continuous column that is pulled up from the roots. Wilting happens when a plant loses water faster than it takes it up, so cells become flaccid.

How water moves up the xylem

Water is lost from the mesophyll cells by evaporation. This pulls more water out of the xylem to replace it. This pull is called the transpiration pull.

The pull is passed down the xylem because water molecules are attracted to each other. These forces of attraction hold the water molecules together as a continuous column of water, so the whole column is drawn up the xylem, from the roots to the leaves.

  1. Water evaporates from the surface of the mesophyll cells and diffuses out through the stomata.
  2. Water is drawn out of the xylem to replace it. This is the transpiration pull.
  3. The water molecules are held together by forces of attraction, so the column of water is pulled up the xylem.
  4. More water enters the root to replace it.
A plant with a column of water in the xylem from the roots to the leaves. In the leaf, water evaporates from mesophyll cells and water vapour leaves through a stoma. Inside a xylem vessel, water molecules are held together by forces of attraction and the column of water is pulled up. Water enters the root hair cells at the bottom, and an arrow shows the transpiration pull. (opens full size in a new tab)
Evaporation from the leaves causes the transpiration pull. Forces of attraction between water molecules hold the column together.

Wilting

Wilting happens when a plant loses water by transpiration faster than it can take water up from the soil. Water leaves the cells by osmosis, so the cells lose their turgor (they become flaccid). The cells no longer press against each other, so the leaves and stems are not supported and droop.

  • Wilting is more likely in hot, dry or windy conditions, because transpiration is fast.
  • Wilting also happens when the soil is dry, because there is little water to take up.
  • When water uptake is greater than water loss again, the cells take in water, become turgid and the plant recovers.
Exam tip:

Explain wilting in terms of cells: water loss means the cells lose turgor (become flaccid), so the plant loses its support and droops.

Quick check

  1. Define transpiration.

    Show answer

    The loss of water vapour from leaves.

  2. Name two factors that increase the rate of transpiration when they increase.

    Show answer

    Temperature and wind speed.

  3. Supplement How does higher humidity affect the rate?

    Show answer

    It slows transpiration, because the concentration gradient is less steep.

  4. Supplement What holds the water column together in the xylem?

    Show answer

    Forces of attraction between water molecules.

Written and checked against the Cambridge IGCSE Biology (0610) specification · Updated October 2026

Frequently asked questions

How does temperature affect the rate of transpiration?

A higher temperature increases the rate of transpiration, so a leafy shoot loses water vapour from its leaves faster. You can show this with a potometer, using rooms or water baths at different temperatures and measuring how far the air bubble moves in a set time. Keep the shoot, light and wind speed the same.

Why does wind increase the rate of transpiration?

Supplement Wind increases the rate of transpiration because it blows water vapour away from around the stomata. This keeps the concentration gradient between the air spaces inside the leaf and the outside air steep, so water vapour diffuses out faster. Higher humidity has the opposite effect, because humid air makes the gradient less steep.

How does a potometer measure transpiration?

A potometer measures how much water a cut leafy shoot takes up. As the shoot takes up water, an air bubble moves along a capillary tube, and the distance moved in a set time gives the rate of water uptake. Most of the water taken up is lost by transpiration, so this estimates the rate of transpiration.

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