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Genetic diagrams, Punnett squares and pedigrees

Inheritance · Monohybrid inheritance · note 2 of 5

Genetic diagrams, Punnett squares and pedigreesSpec 17.4.10, 17.4.11, 17.4.12

In short

A monohybrid cross shows the inheritance of one characteristic controlled by a single gene, using a genetic diagram or Punnett square of the parents' gametes. Crossing two heterozygous parents gives a 3 : 1 phenotypic ratio, and heterozygous crossed with homozygous recessive gives 1 : 1. Pedigree diagrams trace a characteristic through a family to show which allele is dominant.

A monohybrid cross looks at the inheritance of one characteristic controlled by a single gene. You show the result using a genetic diagram or a Punnett square, which lists the possible gametes and the genotypes they can make.

  1. Choose letters for the alleles: a capital letter for the dominant allele and the same letter in lower case for the recessive allele.
  2. Write the genotypes of the parents.
  3. Write the gametes each parent can make. Each gamete has only one allele.
  4. Put the gametes along the top and side of a Punnett square and fill in the boxes to show the possible offspring genotypes.
  5. Write the phenotype for each genotype and work out the ratio.

Two heterozygous parents (3 : 1)

Tall (T) is dominant to short (t) in pea plants. Two heterozygous tall plants are crossed. Show the possible offspring and the phenotypic ratio.

  1. Parents: Tt × Tt.
  2. Gametes: T or t from each parent.
  3. Punnett square boxes: TT, Tt, Tt, tt.
  4. TT and Tt are tall. tt is short.

Answer: Genotypes 1 TT : 2 Tt : 1 tt. Phenotypes 3 tall : 1 short.

Punnett square for Tt × Tt: gametes T and t from each parent give offspring TT, Tt, Tt (tall) and tt (short). (opens full size in a new tab)
Tt × Tt gives 3 tall : 1 short (1 TT : 2 Tt : 1 tt).

Heterozygous × homozygous recessive (1 : 1)

Black fur (B) is dominant to brown fur (b) in guinea pigs. A heterozygous black guinea pig is crossed with a brown one. Show the possible offspring and the phenotypic ratio.

  1. Parents: Bb × bb.
  2. Gametes: B or b from the first parent; only b from the second.
  3. Punnett square boxes: Bb, Bb, bb, bb.
  4. Bb is black. bb is brown.

Answer: Genotypes 1 Bb : 1 bb. Phenotypes 1 black : 1 brown.

Exam tip:

The ratios you need are 3 : 1 and 1 : 1. Circle the gametes, show every Punnett box, and write the phenotype beside each genotype.

Pedigree diagrams

A pedigree diagram (family tree) shows how a characteristic is inherited through a family. Squares are usually males and circles are females. Shaded shapes show people with the characteristic. A horizontal line joins parents and a vertical line leads to their children.

  • Two parents without a characteristic who have a child with it: the characteristic is recessive, and both parents are heterozygous (carriers).
  • Two parents with a characteristic who have a child without it: the characteristic is dominant, and both parents are heterozygous.
Three-generation family pedigree with squares for males and circles for females: an affected grandmother (rr), her carrier son (Rr) and his carrier partner (Rr) have an affected daughter (rr) and two unaffected children. (opens full size in a new tab)
Two unaffected parents with an affected daughter: the characteristic is recessive and both parents are carriers (Rr).
Exam tip:

In a pedigree question, find two parents with the same phenotype who have a child with the other phenotype. That tells you which allele is dominant.

Written and checked against the Cambridge IGCSE Biology (0610) specification · Updated October 2026

Frequently asked questions

What is a genotype and phenotype?

A genotype is the genetic make-up of an organism in terms of the alleles present, such as Tt. A phenotype is the observable features of an organism, such as being tall. A heterozygous Tt pea plant has the tall phenotype, because the dominant allele T is expressed even though a recessive allele is present.

What is a Punnett square used for in genetics?

A Punnett square is used to show the possible offspring of a genetic cross. The gametes of one parent go along the top and the other along the side, and the boxes show the possible offspring genotypes. From these you work out the phenotypes and the ratio, such as 3 : 1 or 1 : 1.

How do you find out if an organism is homozygous or heterozygous?

Supplement You use a test cross: cross the organism showing the dominant phenotype with a homozygous recessive individual. If any offspring show the recessive phenotype, the unknown parent must be heterozygous. If all the offspring show the dominant phenotype, it is probably homozygous dominant. A large number of offspring gives a more reliable result.

All 5 questions on Monohybrid inheritance